Typing of HLA-A, -B and DRB1 antigens in clinical practice in patients of the Rostov population for early diagnosis of rheumatoid arthritis

Objective : a retrospective analysis of the results obtained by typing HLA-A, -B antigens and HLA DRB1 allele groups in patients with rheumatoid arthritis (RA) Rostov population. Materials methods rheumatic inflammatory diseases who were on inpatient treatment rheumatology department SBI “OKB No. 2” was carried out LITT RO "BTS" 2019-2020. 41 (9 men, 32 women, median age — 42 years) diagnosed RA. All typed HLA-DRB1 alleles real-time polymerase chain reaction DNA-TECH reagents (Russia). To isolate DNA, set Sample-Rapid-Genetics used NPO DNA Technology LLC. 39 аccording to -B. Typing standard lymphocytotoxic test DILEN (Czech Republic). Lymphocytes isolated density gradient “Lympholot”, company. The control group consisted healthy residents population (donors hematopoietic stem cell registry). Results showed that DRB1*04 occurs 46.3% RA, 61.1% seropositive RA (control 20%). There decrease frequency DRB1*13 (9.7%) compared (24.4%). Conclusions high level reliability (p<0.001) increase confirms their associative relationship A indicates protective function these are important for early diagnosis